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MetaMorph Inc linescan kymograph images
Membrane nanotubes form connections between MDA-231 cells in culture. ( a , b ) Representative lattice light-sheet image of MDA-231 cells visualized by a GFP-tagged membrane marker, illustrating nanotubes interconnecting with other MDA-231 cells and contacting the coverglass. See also Movie . ( a ) is a maximum intensity z-projection (top view) of the imaging volume, and ( b ) shows an orthogonal (side) view of the same volume. ( c , d ) Histograms plot the distributions of lengths of nanotubes that interconnect MDA-231 cells ( c , n = 98 nanotubes from 16 imaging fields) and those that contact the cover glass ( d ) 113 nanotubes). Insets in ( c , d ) are transverse <t>linescan</t> profiles showing the widths of nanotubes that interconnect MDA-231 cells (n = 6, mean FWHM 466 ± 31 nm) and those that contact the coverglass (n = 6, mean FWHM 423 ± 15 nm).
Linescan Kymograph Images, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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universal imaging inc 1d-kymographs
Membrane nanotubes form connections between MDA-231 cells in culture. ( a , b ) Representative lattice light-sheet image of MDA-231 cells visualized by a GFP-tagged membrane marker, illustrating nanotubes interconnecting with other MDA-231 cells and contacting the coverglass. See also Movie . ( a ) is a maximum intensity z-projection (top view) of the imaging volume, and ( b ) shows an orthogonal (side) view of the same volume. ( c , d ) Histograms plot the distributions of lengths of nanotubes that interconnect MDA-231 cells ( c , n = 98 nanotubes from 16 imaging fields) and those that contact the cover glass ( d ) 113 nanotubes). Insets in ( c , d ) are transverse <t>linescan</t> profiles showing the widths of nanotubes that interconnect MDA-231 cells (n = 6, mean FWHM 466 ± 31 nm) and those that contact the coverglass (n = 6, mean FWHM 423 ± 15 nm).
1d Kymographs, supplied by universal imaging inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Verlag GmbH kymograph analysis software image j
Membrane nanotubes form connections between MDA-231 cells in culture. ( a , b ) Representative lattice light-sheet image of MDA-231 cells visualized by a GFP-tagged membrane marker, illustrating nanotubes interconnecting with other MDA-231 cells and contacting the coverglass. See also Movie . ( a ) is a maximum intensity z-projection (top view) of the imaging volume, and ( b ) shows an orthogonal (side) view of the same volume. ( c , d ) Histograms plot the distributions of lengths of nanotubes that interconnect MDA-231 cells ( c , n = 98 nanotubes from 16 imaging fields) and those that contact the cover glass ( d ) 113 nanotubes). Insets in ( c , d ) are transverse <t>linescan</t> profiles showing the widths of nanotubes that interconnect MDA-231 cells (n = 6, mean FWHM 466 ± 31 nm) and those that contact the coverglass (n = 6, mean FWHM 423 ± 15 nm).
Kymograph Analysis Software Image J, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Membrane nanotubes form connections between MDA-231 cells in culture. ( a , b ) Representative lattice light-sheet image of MDA-231 cells visualized by a GFP-tagged membrane marker, illustrating nanotubes interconnecting with other MDA-231 cells and contacting the coverglass. See also Movie . ( a ) is a maximum intensity z-projection (top view) of the imaging volume, and ( b ) shows an orthogonal (side) view of the same volume. ( c , d ) Histograms plot the distributions of lengths of nanotubes that interconnect MDA-231 cells ( c , n = 98 nanotubes from 16 imaging fields) and those that contact the cover glass ( d ) 113 nanotubes). Insets in ( c , d ) are transverse linescan profiles showing the widths of nanotubes that interconnect MDA-231 cells (n = 6, mean FWHM 466 ± 31 nm) and those that contact the coverglass (n = 6, mean FWHM 423 ± 15 nm).

Journal: Scientific Reports

Article Title: Lattice light sheet imaging of membrane nanotubes between human breast cancer cells in culture and in brain metastases

doi: 10.1038/s41598-017-11223-y

Figure Lengend Snippet: Membrane nanotubes form connections between MDA-231 cells in culture. ( a , b ) Representative lattice light-sheet image of MDA-231 cells visualized by a GFP-tagged membrane marker, illustrating nanotubes interconnecting with other MDA-231 cells and contacting the coverglass. See also Movie . ( a ) is a maximum intensity z-projection (top view) of the imaging volume, and ( b ) shows an orthogonal (side) view of the same volume. ( c , d ) Histograms plot the distributions of lengths of nanotubes that interconnect MDA-231 cells ( c , n = 98 nanotubes from 16 imaging fields) and those that contact the cover glass ( d ) 113 nanotubes). Insets in ( c , d ) are transverse linescan profiles showing the widths of nanotubes that interconnect MDA-231 cells (n = 6, mean FWHM 466 ± 31 nm) and those that contact the coverglass (n = 6, mean FWHM 423 ± 15 nm).

Article Snippet: Linescan kymograph images were derived by measuring fluorescence across an area to encompass all nanotube connecting cells (width 15 pixels, 2.4 μm) from ΔF/F 0 image stacks (created as described above), displaying their evolution over time as a pseudocolored representation utilizing the kymograph function in MetaMorph.

Techniques: Membrane, Marker, Imaging